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The minimum step was 0.13 arcmin.
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The minimum detectable temperature step was 0.1°C.
This was affected by the true turning angle and for a true turning angle of 90° the minimum step length was 1.08 error standard deviations (Figure 5B).
For a true directional bias of 90° this minimum step length was 0.51 error standard deviations (Figure 5D).
Kinetic modeling of differential equations used the Rosenbrock stiff algorithm in the Macintosh computer software Berkeley Madonna (version 8.3.18; copyright Robert I. Macey and George F. Oster); the minimum step size was set to 1 × 10− 6 s and the maximum to 1 s, with a tolerance of 0.01.
Structures were localized under brightfield illumination to minimize loss of fluorescence and subsequently imaged under confocal modes with a minimum pinhole diameter (this step was rigorously followed during live varicosity imaging after FM staining).
If the previous step causes a change in Q →, the adaptive step size is decreased by setting h to h/10 and the calculation is repeated until either the minimum step size is reached or the processing of events and assignment rules does not change Q → anymore.
Protein pellet obtained in each step was dissolved in minimum volume of 100 mM sodium phosphate buffer (pH 7.1), and dialyzed extensively against water using 12 kD membrane (Sigma chemicals) for 24 h.
The sample preparation step was simplified to an absolute minimum.
This top-up and taxing step was repeated until every licence had a minimum of 7t.
The first is to find all itemsets that satisfy the minimum support; this step is generally expensive, due to the requirement for multiple passes over the database (see Fig. 1).
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