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It can be obtained by calculating the theoretical laser minimum spot diameter (D0) as D 0 ≈ 1.27 λ 0 f D, where λ0 is the wavelength of the laser, f is the effective focal length of the lens, and D denotes the laser beam diameter.
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The parameters used in this case were as described in the previous equation; however, the equilibration time, t eq was set to 1.5 × 1010 s, the laser frequency R rep at 100 kHz, the pulse dwell time D t calculated from the effective number of pulses, and finally, the focal area A foc was calculated from the theoretical minimum laser spot diameter.
The minimum laser spot diameter can reach 100 μm with the focus system.
The minimum spot size is 7.5-um-diameter.
A laser spot diameter on a cell is 1 mm.
Spot diameter of laser beam was nearly 1 μm.
The spot diameter onto the sample was approximately 1 μm.
The composition of the silicate portion was determined by 10 randomly selected EPMA spots (10 μm spot diameter, Table 4).
The laser spot diameter was 100 µm.
The spotted volume of this oligonucleotide solution was 200 pl, producing a spot diameter of approximately 220 µm.
When the spot diameter approaches the size of the area "near rat urine" (e.g. the width of the arena), the time visiting this area reaches an asymptotic line at a minimum.
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