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A smaller sample size will be required for a similar precision with a minimum specificity of 75% (n = 52).
Labeled samples were assessed by Nanodrop for sufficient quantity and a minimum specificity of 8, where specificity is the concentration of the dye (pmol/μg) times 1000 divided by the concentration of the cRNA (ng/μL).
In fact, when utilising previous diagnoses (plus anti-asthma medication usage), sensitivity increased to ≥0.8 (≥0.9 when including anti-asthma medication usage variables) at a minimum specificity of 0.9.
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As Messina was supplied a minimum specificity requirement of 90% when these data were analysed, and this probeset could not satisfy this condition, it was rejected by Messina.
When applied to ConoServer and UniProtKB/Swiss-Prot databases, ConoSorter is able to recognize 100% of known conotoxin superfamilies and classes with a minimum species specificity of 99%.
The possibility for cross-reactivity may seem high and even potentially dangerous, but given the number of potential T cell epitopes represented by modifying the 20 amino acids at each of the TCR-facing positions, it turns out that this reflects a minimum cross-specificity of 1 100,000 peptides.
After considering the variation of the herd size and the positive cutoff, the simulations determined that the minimum number of cows that needed to be sampled to ensure a minimum sensitivity and specificity of 95%% was 20 animals.
Only vertebrate transcription factors, with a specificity of minimum 10 bits and a TF score threshold of 90%, in parts of the sequences presenting a minimum conservation of 90% between the species.
Based on this assumption, and a minimum required sensitivity and specificity of 80%, a sample of 300 participants would allow estimation of sensitivity and specificity of self-report versus BCO with 95% confidence intervals within 6.4% of the point estimate.
[ 12] Completion of this national training and calibration based on a minimum sensitivity of 0.75 a specificity of 0.90 for the primary teeth and a minimum sensitivity of 0.80 a specificity of 0.90 for the permanent teeth was used as the main selection criterion for the examiners used in this study.
The completeness of case ascertainment has been previously reported to have a minimum sensitivity of ∼90% and specificity of 99.5% compared with identification of cases by cancer registries (Michaud et al, 2005).
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