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In this paper, we consider the capacitated minimum spanning network (CMSN) problem, which asks for a minimum cost spanning network such that the removal of r and its incident edges breaks the network into a number of components (groups), each of which is 2-edge-connected with a total weight of at most k.
Open image in new window Fig. 2 Minimum spanning network of the mtDNA COI gene sequence haplotypes (579 bp) of Siganus javus.
The minimum spanning network (MSN) (Bandelt et al. 1999) of the haplotypes was constructed and implemented in PopART 1.7 (Leigh and Bryant 2015).
As such, eBURST and a maximum parsimony minimum spanning network analyses were carried out.
Evidence of these events is clear in the star-like shape of the minimum spanning network, the pairwise mismatch distribution, and the skyline plot.
A median joining algorithm was used to build a minimum spanning network to examine the evolutionary relationships among haplotypes, as implemented in Network 4.5 (http://fluxus-engineering.com/).
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In the absence of recombination, all methods recovered the correct topology and branch lengths nearly all of the time when the substitution rate was low, except for minimum spanning networks, which did considerably worse.
Lastly, Arlequin was used to calculate nucleotide diversity and minimum spanning networks [ 51].
The sequence data has also been used to reconstruct neighbour-joining trees and minimum spanning networks (MSNs; Additional file 1, Figures S2 and S3, [ 1]).
We used the software NETWORK 4.111 [ 57] to estimate gene genealogies for each of the nuclear genes and for the combined set of nuclear genes, and to construct unrooted minimum spanning networks.
The median-joining method first constructs the minimum spanning networks before adding a few consensus sequences that function as median vectors in order to arrive at the most parsimonious networks [ 48].
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