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The MAR Finder program algorithm scores A/T nucleotides along promoters using a 200 bp window at 10 bp intervals with MAR potential of 0.1 as minimum significance threshold.
This latter observation is supported by the identification of only three putative SMR sequences from Crenarchaeal genome BLAST searches with e-values at the minimum significance threshold (5 × 10-3).
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98 peptides were not detected in any of the four 8-plex analyses applying our stringent search criteria (Mascot ions score threshold 20, rank 1, Mascot significance threshold 0.05, minimum peptide length 8, maximum peptide mass deviation 8 ppm) that ensured false discovery rates less than 1% on both the peptide and protein levels in all analyses.
In one epigenome-wide association study in the SMART study population (N = 43) [ 53], only two CpG sites in the GSTM1 gene showed increased DNA methylation with elevated blood mercury exposure under the established significance threshold of minimum absolute change of 10% and a P value <0.05.
In one epigenome-wide association study in the SMART study population (N = 35) [ 29], only one CpG site in the TSP50 gene promoter showed increased DNA methylation with elevated BPA exposure under the established significance threshold of minimum absolute change of 10% by BPA levels and a P value <0.05.
The 5% quantile point of the minimum P value represents the genomewide significance threshold at this marker density.
Data was segmented using the SNP-Rank segmentation algorithm with a minimum of 8 probe sets and significance threshold of p-value of 10−6.
Researchers just need to define the analysis parameters, such as the covariates to be used in the ANOVA/MANOVA, the FDR correction method, the significance threshold and the minimum difference between mean methylation values between the groups under investigation.
Candidate gene duplicates obtained in the above GH analyses that had BLAST hits with query and subject coverage of at least 40%, a minimum percent identity of 25% and significance threshold of E ≤ -3 to Repbase V11.05 sequences were excluded from further analysis [ 87, 88].
For each execution mode the user can specify the minimum base quality score, the minimum read quality score and the minimum depth of coverage for the pileup computations and the significance threshold for the statistical tests used in the GENOTYPE and ASE modes (default values set to 0, 0, 1, 5%, and 5%, respectively).
The significance threshold was set to a minimum of 95% (p ≤ 0.05).
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