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To avoid selecting genes with high foldchange due to low signal intensity a minimum signal intensity value was utilized.
MS/MS was performed using an isolation width of 2 m/z, normalized collision energy of 35% and a minimum signal intensity of 1000 counts.
MS/MS was performed using an isolation width of 2 m/z, normalized collision energy of 35%, a minimum signal intensity of 1000 counts, and the dynamic exclusion option enabled.
Analysis was restricted to cDNA elements with a regression correlation of >0.6, fluorescence intensities of >2.5 fold signal/background in Cy3 or Cy5 channels and a minimum signal intensity of >100 in both channels for at least 80% of the arrays.
The minimum signal intensity of a true probe signal was set to 9.0 on a log2 scale and therefore all measured intensities below 9.0 are considered uncertain as these probe values are inseparable from background noise, and the minimum distances of a differentially and a similarly expressed transcript was set to 25 and 36 respectively (for details see Thomassen et al. [21]).
Statistical significance cutoff was set to P < 0.01; minimum signal intensity was set at 50 A. Full data are deposited at NCBI GEO, accession no GSE41800.
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Other mass spectrometric data generation parameters were as follows: collision energy 24%35%5% for MS3 scans), MS scan range 400 1,800 m/ z, minimum MS signal intensity 500 counts, minimum MS/MS signal intensity 100 counts, and MS/MS activation time 120 ms (30 ms for MS3 scans).
The modified, normalized intensity for a gene was derived from its normalized intensity by subtracting the minimum normalized signal intensity across all genes in the sample and adding a value of one (to avoid dividing by zero).
The rice DCL genes OsDCL1a, OsDCL2, OsDCL3a and SHO1 were found to express ubiquitously (minimum normalized signal intensity values 67.7, 290.5, 32.0 and 82.2, respectively) in the tissues/stages of plant development analyzed [see Additional file 2].
Whilst tuning the ICP-MS, compromise conditions for maximum signal intensity of the analyte (Mg+, In+, U+) and minimum ratio of oxide (CeO+/Ce < 3%) and doubly charged ions (Ba2+/Ba+ < 3%) were found.
For each permutation, we recorded the smoothed data's maximum signal intensity and position of the maximum on each chromosome, as well as the minimum and position of the minimum on each chromosome.
More suggestions(15)
minimum signal strength
minimum signal noise
minimum signal attenuation
minimum signal pickup
minimum signal level
minimum probe intensity
minimum signal energy
minimum signal value
minimum frequency intensity
minimum stress intensity
minimum signal amplitude
minimum signal change
minimum saturation intensity
minimum signal deviation
minimum signal requirement
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