Your English writing platform
Discover LudwigExact(3)
Further reductions in error rate were achieved by conditioning on a minimum sequence quality of every SNP (e.g.: 0.07% for a sequence quality of 20).
Initial polymorphism tagging was performed using Polyphred, with a minimum sequence quality of phred 25 (Nickerson et al. 1997).
For example, the probability of eight minor alleles being called incorrectly (e.g., due to sequence artifact) for a certain SNP identified as homozygous in 4 of 17 inbred murine strains by these criteria based on minimum sequence quality (i.e., Phred score) is less than 1 × 10−.
Similar(57)
Threshold values were then applied to obtain the final set of contigs representing European eel transcripts: a minimum contig length of 200 nucleotides and a minimum average sequence quality of 30 (Phred Scores), corresponding to an error rate of 1 per 1,000 bp.
Element sequences were chosen for minimum overlap, sequence quality, and other criteria [ 50].
A further quality check was conducted for the final set of putative transcripts by selecting only those sequences at least 200 nucleotide long and with a minimum average sequence quality of 30 (corresponding to an average error rate of 1/1,000).
Previous studies have applied various rules for sequence selection: sequences must start with a restriction motif [ 8], sequences must be aligned to a unique location in the genome [ 9], and sequences must have a minimum average sequence quality (SQ) score of 20 [ 8, 9].
A further quality check was conducted for the final set of putative transcripts by filtering sequences by length (minimum 200 bp) and average sequence quality (minimum of 30).
All 6,464 sequence-verified FLcDNAs achieved a minimum of Phred30 sequence quality at every base (i.e., no more than one error in 103 bases).
We only kept SNVs meeting stringent quality criteria: 1) Minimum allele quality: accumulated sequence quality for every allele; 2) Minimum mapping quality.
The minimum allele quality (accumulated sequence quality for every allele) was not lower than 20.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com