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Simulation studies showed that >95% of the homoplasmic variants were detected at a minimum sequence coverage of 20× while heteroplasmic variants required >200× coverage.
Hence, the final assemblies were based on a minimum sequence coverage of 3×.
Final assemblies from the FLX and GLS platforms were based on minimum sequence coverage of 59 ×.
Alignments with a minimum score identity of 90% and a minimum sequence coverage of 80% were filtered out.
Different hss and bph parameters leading to the best assemblies of different BACs indicated that at a given minimum sequence coverage individual features like repeat content and/or composition may essentially influence the quality of the assembly.
To minimize this false negative rate (FNR) we provide a list of SNPs and INDELs using a less stringent calling criterion (5X minimum sequence coverage; Additional file 5, Table S3).
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The minimal sequence coverage was set to a minimum of 10%.
Such information is invaluable because it allows us to determine the minimum sequencing coverage required before even beginning an experiment.
Among the tumor samples, we obtained a minimum sequencing coverage of 100 × for 85.7% of the exonic-targeted regions.
A certain minimum sequencing coverage is necessary for methylome analysis and several recent studies have demonstrated the advantages of the use of SMRT sequencing technology [ 44– 44].
All missense, nonsense, and indel mutations were included in our analysis if present in at least 5% of reads with a minimum sequencing coverage of 10.
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