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The minimum sequence complementarity and thermodynamics of hybridization required for a region of mRNA to serve as a miRNA target site in vitro are not fully understood.
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The target cleavage requires extensive sequence complementarity and dramatically accelerates core-RISC recycling.
The last feature was that of sequence complementarity represented mathematically as relative thermodynamic stability.
This is achieved through binding of sRNAs by partial nucleotide sequence complementarity to their target mRNA sequences.
Sense and antisense transcripts are defined as a pair of RNA molecules with significant sequence complementarity [1], [4], [5].
A classical hypothesis for seed sequence complementarity is that the miRNAs of C19MC, like all other miRNAs target mRNAs.
24 Seed sequence complementarity is critical for effective miRNA targeting.
In animals, miRNAs target mRNAs with partial sequence complementarity [ 50].
MiRNAs bind to their target genes based on sequence complementarity.
The targeting depends on either complete sequence complementarity for inducing transcript degradation or partial sequence complementarity for translational repression.
Usually high sequence complementarity beyond miR and miR* sequences indicate their recent evolution.
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