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Alignments with a minimum score identity of 90% and a minimum sequence coverage of 80% were filtered out.
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To estimate the degree of genome coverage a total of 90,955 ESTs were mapped to the draft 2 assembly with the program BLAT [ 32] using stringent alignment parameters (minimum score = 400, minimum identity = 98%).
The BLAST parameters were set as follows: BLAST cutoff e-value was 1 e − 5; homologs were selected at e-value of 1 e − 8; identity homolog was 0.95; the blast minimum score was 30; and minimum identity was 90.
The minimum score for BLAST BLAST SS) and minimum percent identity (BLAST % identity) were set at the default 0. The maximum BLAST high-scoring segment pairs (HSP) length was set at 1000.
The mouse genome sequence had already been soft-masked for repeats by UCSC and BLAT was set to produce all possible alignments (tile size = 10, minimum score = 0, minimum sequence identity = 0).
Previously, there was no minimum score.
"I'd say 700 is a minimum score now.
Minimum score = −8, Maximum score = 4.
What was the minimum score I could get in my final exam and still get the score I needed?
Out of these scores the minimum score smin is selected.
Minimum score is 20.
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CEO of Professional Science Editing for Scientists @ prosciediting.com