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Despite the device requiring a minimum sample volume of 1·2 l, eight of the nine subjects who failed had a forced vital capacity (FVC) of more than 1·5 l.
These differences might be related to the fact that for MR imaging, a minimum sample volume of approximately 1 mm3 is needed; this is larger than the sample volume needed for histological studies.
The minimum sample volume is 2 mL per time point.
The method requires a minimum sample volume of 250 μl and has a detection limit of 10 pg/ml.
Compared with these assays, dried blood spot (DBS) analysis provides several advantages, including a minimum sample volume required for the measurement of drugs in whole blood.
In order to meet the minimum sample volume requirements of the IL-6 assay, dialysate samples were diluted 100-fold with calibrator diluent.
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These platforms explored the detection of ultra-low concentrations of target biomarkers and have realized rapid, ultrasensitive and cost-effective bioassays requiring minimum sample volumes, which will enable primary care physicians and patients to perform assays in their respective settings, using so-called point-of-care diagnostics.
By reducing the volume of fluid in the sample application lines and the flow cell, flushing requirements to achieve clean baselines and minimum sample volumes are reduced.
This surface modification combined with the microfluidic system of the SPR instrument made it possible to test a panel of capturing agents in parallel with minimum sample volumes (20 μl).
The following general formula may be used to calculate the minimum sampled volume: V s=frac{20}{Cn, m}times 1000.
The sample homogeneity was assessed by a one-way analysis of variance (ANOVA) [29], and finally the F test values (Fcal) were calculated to be lower than the critical values at 95%% confidence for all 3 candidate plasmid between- and within-bottle (Additional file 1: Table S1), which clearly demonstrated their good homogeneity with the minimum sampling volume as low as 1 μL.
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