The minimum repeat unit was defined as 12, 6, 4, 3, 3 and 3, respectively, for the mono- to hexanucleotide.
Microsatellites were defined considering the minimum repeat unit as six for di- and five for tri-, tetra-, penta-, and hexanucleotides.
The minimum repeat unit length was set to 2 and the maximum repeat unit length to 4. The minimum microsatellite length was set to 24.
The eTandem algorithm was run with a minimum repeat unit of 2 bp and a maximum repeat unit of 100 bp, as previously described [ 26].
The parameter settings were as follows: the minimum repeat unit was defined as seven repeats for dinucleotide motifs, five repeats for tri-motifs, four repeats for tetra-motifs, and three repeats for penta-, hepta-,hepta-, and octa-motifs.
The minimum repeat unit was prescribed as follows: 10 repeats for mononucleotides, 6 for di-nucleotides and 5 for all the other motifs such as tri-, tetra-, penta- and hexa-nucleotides.
Di- to hexa-nucleotide SSRs with a minimum repeat unit size of five (for tri- to hexa-nucleotide) or six (for di-nucleotide) were identified based on the analysis of assembled isotig templates.
The size of motifs was two to six nucleotides, and the minimum repeat unit was defined as six for di-nucleotides and four for tri-, tetra-, penta-, and hexa-nucleotides.
To facilitate SSR detection, only 1- to 6-nucleotide motifs were considered, and the minimum repeat unit was defined as 10 for mono-, 6 for di-, and 5 for tri-, tetra-, penta-, and hexa-nucleotides.
The size of motifs was one to six nucleotides, and the minimum repeat unit was defined as 10 for mononucleotides, five for dinucleotides, and four for tri-, tetra-, penta-, and hexa-nucleotides.
