The minimum repeat unit was defined as 12, 6, 4, 3, 3 and 3, respectively, for the mono- to hexanucleotide.
Microsatellites were defined considering the minimum repeat unit as six for di- and five for tri-, tetra-, penta-, and hexanucleotides.
The minimum repeat unit length was set to 2 and the maximum repeat unit length to 4. The minimum microsatellite length was set to 24.
The eTandem algorithm was run with a minimum repeat unit of 2 bp and a maximum repeat unit of 100 bp, as previously described [ 26].
Di- to hexa-nucleotide SSRs with a minimum repeat unit size of five (for tri- to hexa-nucleotide) or six (for di-nucleotide) were identified based on the analysis of assembled isotig templates.
The minimum repeat unit was prescribed as follows: 10 repeats for mononucleotides, 6 for di-nucleotides and 5 for all the other motifs such as tri-, tetra-, penta- and hexa-nucleotides.
The minimum repeat units for MNRs (mononucleotides repeats), DNRs (dinucleotide repeats), TNRs (trinucleotide repeats), TTRs (tetranucleotide repeats), PNRs (pentanucleotide repeats), and HNRs (hexanucleotide repeats) were chosen to be 10, 6, 5, 5, 5, and 5, respectively.
Motif size search ranged from mono- to tridecanucleotides, with minimum repeat units set as follows: mononucleotides ≥10; di-, tetra-,etrand penta-, and hexanucleotides ≥5; the remaining repeat motifs ≥5.
Using the computer program MISA (Thiel et al., 2003), the reference contigs were scanned for perfect di-, tri-, and tetranucleotide motifs with minimum repeat units of eight, six, and five, respectively.
A total of 1191 perfect microsatellite repeats were identified from the contigs using the computer program MISA (Thiel et al., 2003), including 809 di-, 291 tri-, and 91 tetranucleotide repeat motifs, with minimum repeat units of 8, 6, and 5, respectively.
This means that it is sufficient to consider the atomic arrangement only in of the unit cell, which is the minimum repeating unit of the [11 0] projection, to fully describe the crystal structure when viewed along the [11 0] direction.
