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REPuter was used to visualize both forward, palindrome, reverse and complement repeats, with a minimum repeat size of 30 bp and a sequence identity greater than 90% with hamming distance equal to 3 in P. chekiangensis and P. bournei (Kurtz and Schleiermacher 1999).
For repeat identification, the following constraints were set to REPuter: (i) minimum repeat size of 30 bp, and (ii) 90% or greater sequence identity, based on Hamming distance of 3 [ 49].
Among the pea ESTs, 118 potential SSRs, with more than five repeat units or a minimum repeat size of 20 nucleotides, were identified in 112 contigs of pea with the program SSRIT (Temnykh et al., 2001; Appendix S1).
For repeat identification, the following constraints were used: (i) minimum repeat size of 30 bp, and (ii) 90% or greater sequence identity, based on Hamming distance equal to 3 bp [ 3].
We submitted the concatenated BAC sequences from each species to RepeatFinder using default parameters except for the following: Block Size = 2000, Minimum Repeat Size = 20, Maximum Repeat Size = 700.
For repeat identification, the following settings were used: (i) minimum repeat size of 30 bp; (ii) 90% or greater sequence identity, based on Hamming distance equal to 3; and (iii) an e-value of 2 for Blast comparisons against the other genomes.
Similar(52)
SSRs are identified using a custom Perl script using minimum repeat sizes of 10 mononucleotides, 5 dinucleotides and 4 tri-, tetra-, penta- and hexanucleotides.
Di- to hexa-nucleotide SSRs with a minimum repeat unit size of five (for tri- to hexa-nucleotide) or six (for di-nucleotide) were identified based on the analysis of assembled isotig templates.
REPuter [ 58] was used to identify the number and location of direct, reverse, and palindromic repeats of genomes with minimum identical repeat size of 20 bp.
The minimum repeat-unit size for di-nucleotides was set at six and at five for tri- to hexa-nucleotide repeats.
Advanced search module can filter repeats of a particular size (mono, tri, tetra, etc.,); can get repeats of a particular pattern (CAG, Poly A etc).; can set the minimum repeat number of each motif size; and can filter repeats of only coding or non-coding regions.
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