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Colors refer to the minimum read depth per sample.
SNP calls were made with SAMtools62 mpileup (version 0.1.18), requiring a minimum read depth of 5 and at least one read on each strand.
SNPs were filtered using maq.pl SNPfilter with minimum read depth (-d) of 20, minimum consensus quality (-q) of 20, and minimum adjacent consensus quality (-n) of 20.
For our analysis we used the default settings of each program, which for 'galign' required a mutant/wild-type reads ratio of 3, and a minimum mutant read of 2. For 'Maq', we used the "SNPfilter" function with its default settings requiring a minimum read depth of 3 reads to identify what the software considered reliable reads.
Consequently, the minimum read depth parameter was fixed to 60.
SNPs were called with a minimum read depth of 3 as a requirement.
Variants detected by GATK were filtered for a minimum read depth of 50.
High confidence variants must have a minimum read depth of 30X and 0.1 maf.
Additional file 7: Coverage at 100x minimum read depth and effect on coverage at 25 and 30 PCR cycles.
The reference genome coverage of all the strains ranged from 86 - 94% with minimum read depth of 10.
All SNPs were filtered by minimum read depth to avoid the analysis of false SNPs due to sequencing errors.
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