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For label-free proteins quantification a minimum ratio counts of 2 was considered and the "LFQ intensities" which are the intensity values normalized across the entire dataset were used.
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Unique and razor peptides were considered for quantification with a minimum ratio count of 1. Retention times were recalibrated based on the built-in nonlinear time-rescaling algorithm.
Proteins with a minimum ratio count of three per group were taken forward for analysis and protein ratios were normalized to the median ratio for each sample and log2 transformed.
Identifications were filtered at a 1% FDR at the peptide level, accepting a minimum peptide length of 7. Quantification required a minimum ratio count of 2. Requantification was enabled, and match between runs was allowed within a 5-min window.
Quantification via normalized H/L ratios was based on minimum of 3 peptide ratio counts.
Users had to maintain a minimum ratio of material uploaded to downloaded.
There's only one doctor per 1,700 citizens in India; the World Health Organisation (WHO) stipulates a minimum ratio of 1 1,000.
The minimum ratio was defined as an individual phenotype.
Parameters established for blast were: minimum identity: 25%; minimum ratio avec query: 60%; minimum ratio avec target: 50%.
Set a minimum ratio.
Mass spectra were processed using MaxQuant 1.3.0.5 (Cox & Mann, 2008; Cox et al, 2011) using default settings, except that ratio count was set to 1, minimum peptide length was set to 6, and only unique peptides were used for quantitation.
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