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First, the sequences were filtered using a minimum quality cut off value of 40.
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‡Discarded reads were removed using Trimmomatic based on a sliding window quality cut off of (4:15) and a minimum length of 36 nt after adapter removal.
Expect values (E values) of 0.001 and bit scores of 30 were used as the minimum allowed cut off.
These two data sets were used to mine the protein sets of each sequenced Lachnospiraceae genome using USEARCH 4.0.38 (Edgar 2010) with an e-value cut off of 10− and a minimum identity cut off of 70%.
As a minimum cut off for group comparisons, and 0.9 for individual comparisons.
The filter criteria used here included a Q20 quality cut-off, estimated copy number of flanking sequences (<2), minimum distance between any two SNPs (≥5 bp) and overall depth (≤100) at a given position in the reference.
The minimum read percentage with non-reference nucleotide is set universally for each gene and each library once the quality cut-off is set, while the sequence depth for each gene and library may vary so that for a certain genes or libraries the true mutation calling may be mixed with noise.
The raw data were quality-filtered using a quality cut-off value of 40.
Minimum generator temperature required to operate the system that is cut off temperature has been evaluated.
The final question was used to cut off the executions that had low quality.
Cut off any excess board so you are left with the minimum allowable for your current puzzle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com