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The stationary background is effectively subtracted away using a minimum projection image taken over the entire image stack (Fig. 4b-d).
For log-transformed images, the minimum projection image across time provides an estimate of the stationary background image B ij. Background-free pathline images were generated by taking the maximum projection across time of I ij mΔt)- B ij. Pathline imaging can be enhanced to color-encode time [ 4, 6].
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A maximum projection image was then produced and duplicated.
The final SWI consists of four sets of images: magnitude images, filtered phase images, minimum intensity projections images and the final SWI images.
A minimum projection value (MPV) was calculated using the average projection for x > 0.5.
Back projection image was obtained after Gaussian filter was applied.
The reconstructed projection image is shown in Fig. 2(d).
Each projection image was recorded in 9 s.
The images are integrated in Z direction and Max XY projection image appears on the screen.
For the projection image analysis, images were collected on a JEOL-JEM2100 at 200 kV.
Care was taken to ensure the same projection image was on the screen in all images.
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