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In addition, Agilent guidelines recommend 150-200 bp minimum probe spacing for optimum probe hybridization.
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The array comprises 385,585 probes semi-tiled across the genome at a 4 bp interval spacing with a minimum probe length of 45 bp, and a maximum length of 85 bp.
The parameters for defining CNAs were as follows: significance threshold = 5.0E-4; maximum contiguous probe spacing (Kbp) = 1000; minimum number of contiguous probes per CNA segment = 5; threshold of signal intensity ratio >0.2 on log2 scale for gains and < −0.3 on log2 scale for losses.
The parameters for defining CNVs were as follows: significance threshold = 1.0E-6; maximum contiguous probe spacing (Kbp) = 1000 Kb; minimum number of contiguous probes per CNV segment = 5; threshold of signal intensity ratio > 0.2 on the log2 scale for gains and < -0.2 on the log2 scale for losses.
OligoWiz 2.0, which is applied here, is an oligonucleotide selection software with several user defined parameters; ΔTm, homology, low-complexity, position and "GATC" only, probe spacing and a maximum and a minimum probe number per sequence.
The minimum detectable CNV length was ∼1 Kb, corresponding to the probe spacing on our array (median 1,136 bp), and the use of SW-ARRAY and permutation testing to assess the significance of any CNV call; in practice the majority of CNVs were longer than this (10th percentile 7.9 Kb).
When starting with intact total RNA samples there is no need for contiguous probe coverage of the target, rather a probe spacing of 20 nt less than the minimum anticipated library insert size, or closer, will suffice to obtain even and continuous depletion.
The sheet resistivity was measured using a four-point probe technique with 3-mm probe spacing.
cylinders except the curing condition and the probe spacing.
F SP is the probe spacing correction factor.
A related probe spacing correction method for non-inline deflected probes was also presented.
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