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A 5-week lithium treatment significantly reduced the plaque number and the minimum plaque area (Fig. 5 B,D).
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Furthermore, in these mice the minimum Aβ plaque area, represented by the small and round-shaped plaques, which might consist of amyloid plaques in formation, was significantly reduced by lithium treatment, once again indicating that lithium treatment prevents rather than removes/dissolves Aβ deposits.
Quantitative analysis of amyloid burden (Fig. 4 E,F) showed a significant lithium treatment effect in the total Aβ plaque area, in the plaque number and in the minimum and maximum plaque areas, both in the neocortex and hippocampus.
Lumen area was inversely proportional to the plaque area.
Average vessel area (VA), plaque area (PA), and LA were measured.
The plaque area in the proximal aorta was measured as percent lesion area per total area of the aorta.
Carotid ultrasound measurements were performed to determine total plaque area (TPA) in both carotids.
Plaques with <50% calcified plaque area were classified as mixed.
Total plaque area and composition of plaque were measured, including initial as well as advanced plaque area.
Number of macrophages per plaque area was determined and individual macrophage area was calculated using number of macrophages per total plaque area.
The visually confirmed threshold was set at 0.005% (plaque area per area of interest).
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