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The minimum pipetting volume used was 10 μl.
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This step is time-consuming and prone to error (due to pipetting volume variations and potential sample mix-ups).
The precision profile is described in terms of possible error sources such as the pipetting volumes of the analyte, enzyme-conjugated antigen, antibody and substrate solutions, calibration curve and inherent absorbances between the wells in an ELISA plate.
Amplified fosmids from all 96 wells on each plate were pooled using a multiwell pipet (volume 100 µL/well), and diluted 1∶1 with LB broth.
The pipetted volume was estimated, from the on-screen display of the droplet dimensions and shape, to be ≈100 nL.
* Minimum volume OPS minimum volume procedures for complex oesophageal interventions were conducted by 297 hospitals.
Their study suggested a minimum annual procedure volume of 13 both regarding hospital volume and surgeon volume.
Minimum centre volume is 400 cases/year.
Maximum LA volume, minimum LA volume and LA volume before contraction were obtained.
FRC is the minimum lung volume under normal breathing.
The minimum sample volume is 2 mL per time point.
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