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The Ribosomal Database Project Naïve Bayesian rRNA classifier was used with a minimum percent identity threshold of 60% for taxonomic assignment (Li et al. 2014).
Clustering was performed using the following variable parameters: minimum percent identity for overlaps (94%), minimum overlap length (30 nt) and maximum length of unmatched overhangs (30 nt).
Three main parameters are provided: the minimum percent identity, merge threshold (bp) and link threshold (bp).
Reads were mapped back to the unigenes with a minimum percent identity = 0.9 and minimum length = 0.5.
Assembly at a lower minimum percent identity resulted in contigs that included sequences from different homeologous gene copies.
The specific parameters used for the analysis were: E-value: 1e-05; minimum percent identity: 30%; algorithm: by present/absent homologs; minimum taxon percent with homologs: 100%.
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A minimum percent nucleotide identity threshold of 70% and 20 bp as a minimal length criterion were considered significant in VISTA [ 18] for our analyses.
For instance, a user can adjust settings such as minimum percent genome identity, merging of links/ribbons according to MT, and the removal of links according to the user-defined LT through the provided online form.
Apart from that, PGC provides users with options for adjusting settings such as minimum percent genome identity, merging of links/ribbons according to MT and the removal of links according to the user-defined LT through the provided online form.
For initial genome sequence pairwise alignment, a default setting value of 70% was used as the minimum percent conservation identity that must be maintained over the window size 11 for a region (> 50 bp) to be considered conserved.
PGC allows users to configure parameters, such as (i) minimum percent genome identity, (ii) link threshold (LT; bp), which removes the links according to user-defined value and (iii) merge threshold (MT; bp), which allows merging of links based on user-defined value.
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