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At around 200 Å, a minimum peak height which was nearly the same as that of the pristine sample was observed.
A band-pass Butterworth filter (4th order) was applied with cutoff frequencies of 1 and 8 Hz, and assuming a maximum stride frequency of 3 Hz, a peak detection function was used to detect peaks with a minimum duration of 0.33 s between peaks and a minimum peak height of 0.5 g.
The parameters for the GPCR algorithm were: object scale = 30 and minimum peak height = 10.
The data analysis was carried out using the calculation for relative crystallinity index [22] of: RCI = I002−Iam/I002×100, where I002 is the maximum peak height above baseline at approximately 22.5° and Iam is the minimum peak height above the baseline at ∼18°.
For automated "base calling" the minimum peak height was set to 100 (in practice most samples gave peak heights well above 1000).
The instrumental limit of detection (LOD) was set as a minimum peak height of 50,000 relative response units due to bad peak shape below that threshold.
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The height distribution shows significant annual variation, with minimum (maximum) peak heights and maximum (minimum) height widths in early Spring (mid-summer).
We ignored peaks with height less than the minimum detectable peak.
Nevertheless, the average peaks heights differed between alleles.
Cross-peak heights were used for NOE distance calibration.
Minimum carotid peak velocity.
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