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However, short partial adapter sequences, which often occur at the ends of reads, are inherently unable to meet this minimum overlap requirement and therefore are not detectable.
Sixth, sequences were collapsed based on gapped alignments, again allowing any extent of overlap meeting the minimum overlap requirement of that assembly job.
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Approximately 75% of re-annotated gene models could be assigned a Gene Ontology term [ 16].> -wrap-foot> Four different minimum overlap requirements (expressed as a fraction of the Chiifu reference gene model) used to compare two gene models at both gene level and exon level.
Minimum overlap required between DMRs = 100 nt.
The best-matching read was chosen, and the other reads were aligned to this best-matching read, with a requirement of 95%% similarity in the overlapping region, which had a minimum overlap of 20 bp.
These parameters include a minimum overlap of 40 bp and a minimum overlap identity of 90%.
The assembled parameters were minimum overlap identity 98% and minimum overlap 50 bp.
A minimum overlap of 25 bp and a minimum %ID of overlap of 95%% was used in the secondary assembly.
The assembly parameters were a minimum overlap length of 40 bp and a minimum overlap identity of 95%.
The assembly parameters used were minimum overlap length of 40 bp and minimum overlap identity of 95%.
The minimum length requirement ensured the CFs, which were found in the control region, did not overlap with those in the flanking region of the PAS.
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