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The minimum output contig size set to 200 bp with the scaffolding option switched off; all other program settings were left unchanged.
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Supplementary Movie 3 showing the reversible stretchability up to 300% with minimum output power decrease.
minimum output energy.
However, the single output contig was composed entirely of keratin mRNA sequence, with no additional contigs deriving from other transcripts.
We first attempted to conduct the GATK analysis with the CLC output contig alignment.
Output contigs shorter than 54nt were removed throughout the assembly with the flag "-lenf 54 0".
We required Oases to output contigs with 100 bases or more.
Early discussions are needed to decide on the purpose, scope and minimum outputs.
Minimum output/retained contig sizes were increased through the assembly using the flags "-lenf 60 1 -lenf 70 5 -lenf 80 20".
CLC-BIO assembly parameters were: minimum contig length = 300; minimum distance = 200; max distance = 600 for Illumina package; minimum contig length = 300 for 454 large package; and minimum contig length = 300; minimum distance = 5,000; max distance = 15,000 for 454 small package.
The average number of contigs per scaffold was 2.05 with a minimum of 1 contig in a scaffold and maximum of 19 contigs in a scaffold.
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