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The simplest approach is to convert the non-positive rates to a number below the minimum observable growth of 1 mm in 5 years (Condit et al. 1993a, 2006).
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The minimal inhibitory concentration (MIC) was reported as absence of no observable growth by the lowest concentration of tested compounds after twofold serial dilution.
A selection of voxel sizes (Fig. 5a) revealed a minimum observable voxel volume to be between 0.25 and 0.68 mm3.
Similar preliminary results were obtained with the simpler Wenglor photoelectric counter, despite the minimum observable diameter being 200 μm and the particle size distribution unavailable.
A total of 12 independent transgenic lines were produced but none of them exhibited any observable growth alteration.
At Figure 3 it can also be seen, that the majority of the observed changes cluster around 0 with 75% having a change in FMD of less than 3%, our stated minimum observable difference.
Increasing the number of participants, unsurprisingly, reduces the minimum observable effect sizes.
Minor but observable growth delays and increased survival was demonstrated (Additional file 1: Figure S5).
It is thus possible to compare predictions of minimum observable effect sizes from the calibration experiment with the actual effect sizes obtained in the main study.
Data from these calibration experiments are sufficient to make estimates of statistical power and predictions of sample size and minimum observable effect sizes.
Predictions of power, sample size and minimum observable effect size (difference in group means) are available across a range of common study designs.
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