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Extended Data Fig. 9 Defining the minimum number of cells required for assessing complex cellular behaviours using Record seq and differential expressed signature gene analysis.
Extended Data Fig. 8 Defining the minimum number of cells required for assessing complex cellular behaviours using Record seq and PCA.
It has been found that the minimum number of cells can be achieved using the second approach.
The results point out that the cell design voltage is higher than the cell voltage at which the minimum number of cells is obtained for the SOFC stack.
In comparison with previous QCA designs, the proposed layouts are implemented with the minimum area, minimum number of cells and delay without any wire-crossing techniques.
Experiment suggests a practical isolator design could limit the minimum number of cells to three or four to ensure a pronounced stop band attenuation effect.
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The untreated cells (negative control) of HepG2 and HeLa cells show a normal morphology (Spindle shape/elongated cells) that adhered to the culture plate with no or minimum number of cell death In contrast to the HepG2 and HeLa cells, the CC1 cells does not show any significant morphological changes even at a concentration of 500 µg/mL (Fig. 4).
Since total assay throughput is limited by image acquisition speed, we utilized a derivation of Devore [27] to estimate the minimum number of cell measurements needed per well to achieve significance (Methods).
Using the total number of cells in each colony to calculate the minimum number of cell divisions that occurred during colony-formation, the frequency of non-binomial DM segregations were approximately 3% (3/88) in CHP-212, 2% (2/100) in MC-IX, and 8% (6/73) in LA-N-5.
A size class defines the minimum number of cell divisions required between the induction of a clone and its formation to the observed size.
This enables us to determine the minimum number of cell equivalents that can be detected by the antibody in question, and when compared with the signal obtained after RNAi, enables us to estimate the degree of silencing obtained.
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