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The Network 4.5.0.1 software package (http://www.fluxus-engineering.com) was used to construct the minimum mutation network by means of the median-joining algorithm [ 22, 23].
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Finally, we show that for mutation landscapes, at equilibrium, with constant environment, there is not necessarily selection for the minimum mutation rate.
In each subset, we required a minimum mutation frequency of 5%.
Step 2: A mutation network and an expression network are constructed based on the mutation and expression matrices, respectively.
For single nucleotide polymorphism (SNP) analysis, a minimum mutation threshold of 3% was applied.
An integrative network M can be obtained by synthesizing the expression network EN and the mutation network MN.
Based on the mutation matrix A generated from somatic mutations and CNVs, a Mutation Network (MN) can be constructed.
Among the overlap mutations, the minimum mutated allele frequency in either DNA or mRNA was 8%.
A minimum spanning network reconstruction was manually drawn by minimization of the number of mutations between haplotypes.
The minimum network represented the minimum number of reactions required to produce the plumage compounds from dietary carotenoids of each species.
A minimum Manhattan network on T is a Manhattan network of minimum possible length.
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