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To search for conserved motifs, we extracted 250 bp of sequence (from −200 to +50 with respect to the translational start site) for the genes in each cluster, and used MEME [79], with the following parameters: distribution of motif occurrences: zero or one per sequence; minimum motif width: 6; maximum motif width: 25.
Range of motif widths was set to 4 and 20 as the minimum motif width and maximum motif width, respectively.
The MEME parameters were set to a minimum motif width of 15 bp and a maximum width of 40 bp.
The parameters were set as follows: maximum numbers of different motifs, 30; minimum motif width, 6; maximum motif width, 50.
Parameters employed in the analysis were as follows: maximum number of motifs, 20; minimum motif width, 6; and maximum motif width, 50.
Parameters were set as follows: the maximum number of motifs 30; minimum motif width 10; and maximum motif width 30; all other parameters were defaulted.
Similar(46)
Highly conserved regions in 56 protein sequences of Mutator and IS256 were detected using MEME, with the following parameters: number of different motifs = 15; minimum and maximum motif width = 5 and 300 amino acids, respectively.
Conserved motifs were detected using MEME with the default settings, except that the maximum number of motifs was defined as nine, and the minimum and maximum motif width was set to 20 and 150, respectively.
MEME was performed with the following settings: any number of repetitions for a single motif distributed among the sequences, the minimum and maximum motif width of 8 bp and the maximum number of motifs to find was 100.
MEME was run with a minimum and maximum motif width of 4 and 50, respectively, and set to return the best 30 motifs.
We looked for zero or one occurrence of each motif among the three positive sequences, with maximum motif width 10 bases and minimum width 5 bases.
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