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For MEME, a fixed minimum motif length of 5 and a maximum of 10 was set and 20 motifs were requested using the zero or one occurrence per sequence model.
Remaining DMFS parameter settings were: minimum motif length l = 5, maximum motif length m = 10 (with both DNA strands being searched); and at most M = 2 mismatches, when aligning elicited motifs to classification set sequences.
To allow identification of DR elements and its spacing and flanking nucleotides, the minimum motif length was set between 12 (length of two half sites with no spacing in between) and 20 nucleotides (length of two half sites with up to 8 nucleotides in between).
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Users select the number of motifs to find per cluster, within a minimum and maximum motif length (6 20 nt).
The parameters were as following: minimum SSR motif length of 10 bp and repeat length of mono-10, di-6, tri-5, tetra-5, penta-5, and hexa-5.
Simple sequence repeats (SSRs) were identified using MISA (http://pgrc.ipk-gatersleben.de/misa/misa.html) with the following parameters; minimum SSR motif length of 8 bp and repeat length of mono-8, di-4, tri-4, tetra-3, penta-3 and hexa-3.
The equal probabilities are assigned to any K with a range between the predetermined minimum and maximum motif lengths, K min and K max.
The parameters were 33 nt maximum motif length, loop size 1 or larger, and minimum length of G-runs at two or three (2G or 3G QFP, respectively).
The program was set to output 7 motifs with minimum and maximum motif length of 10 and 25 bp, respectively.
Therefore, the paired numbers representing SSR motif length and the minimum repeat number in the MISA configuration file (misa.ini) were modified to 2 7, 3 5, 4 5, 5-5 and 6-5 (mono-type excluded).
The mean repeat count in simple and compound microsatellites was highest for mononucleotide motifs and decreased with increasing motif length, as expected from the minimum length thresholds necessary to complete a locus of at least 15 nucleotides.
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