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Exact(4)
The minimum molecule length is set to 150 kb.
Thus in the second round of selection, S t r i c t- T parameter was used for two further assemblies that had relaxed minimum molecule length (R e l a x e d- M i n l e n) of 100 kb rather than the 150 kb default or a strict minimum molecule length (S t r i c t- M i n l e n) of 180 kb.
If one of these runs does not produce a satisfactory assembly, then two minimum molecule length variants (180 and 100 kb) are tested with the p- V a l u e T h r e s h o l d of the current best assembly (Fig. 2 g).
Because neither of the assemblies using 100 or 180 kb as the minimum molecule length improved both assembly metrics when compared to the S t r i c t- T assembly, generated in the first round of selection with the default Minlen of 150 kb, the S t r i c t- T assembly will be referred to as the T. castaneum consensus genome maps in further analysis.
Similar(56)
Molecule maps are filter for minimum molecule lengths of 100, 150 or 180 kb.
The best "-T" parameter was used for two additional assemblies with either relaxed minimum molecule map length (relaxed-minlen) of 100 kb, rather than the 150 kb default, or a strict minimum molecule map length (strict-minlen) of 180 kb.
We generated ∼250x coverage of the T. castaneum genome for single molecule maps >150 kb, the default minimum molecule map length.
The high sensitivity of the duplex ARMS-DCA approach and the short minimum template molecule lengths required for amplification (2706G: 50 nt; 7028C: 68 nt; Figure 1) along with the wide analytical window and the ability to detect mixtures, rendered the ARMS-DCA assay a good candidate for the analysis of samples frequently encountered in forensic genetics.
Figure 8 Critical unfolding temperature ( T unfolding ) versus molecule length.
The conductance values decrease with the increasing of molecule length.
The HA molecule length influences cellular responses.
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