Exact(1)
Since the read length averaged 150 bp and the minimum mapping fraction was 0.5, a breakpoint can have up to 75 bp of sequence data.
Similar(59)
Then, we chose the mapping solution with minimum mapping costs.
At this level there are 14,000 mapping units with a minimum mapping unit of 11 km2.
The spatial resolution of vector-based maps is given by a Variable Minimum Mapping Unit (VMMU), which is class-specific.
a contigs were selected with minimum length of 300-bp and minimum mapped reads of 50.
Spectral transform approaches, namely principal component analysis, band ratio and minimum noise fraction were used for mapping hydrothermally altered rocks and lithological units at regional scale.
The resultant clean and high quality reads were mapped by CLC Genomics Workbench (using the minimum similarity fraction of 0.98, the minimum length fraction of 0.8 and the maximum number of hits of 10) against the improved apple reference transcriptome [ 25], which is available at URL http://www.rosaceae.org/species/malus/malus_x_domestica/CU_RNA_seq_genes of GDR [ 26].
To obtain the gene expression values, reads from each sample replicate were mapped to the genes (i.e. assembly contigs) with the default mapping parameters {minimum similarity fraction = 0.8 and minimum length fraction (long reads) = 0.9} [ 75].
Default settings were utilized except for the following modifications: the minimum intron length was set to 50 nucleotides, the maximum intron length was set to 250 000 nucleotides, the minimum isoform fraction was set to 0.10, and only uniquely mapping reads were maintained (max-multihits was set to 1).
The "RNA-Seq analysis" option was used to map reads to each database at the following settings: minimum length fraction 0.9, minimum similarity fraction 0.8, and maximum number of hits for a read 30.
To get RNA-seq quality metrics, bedtools utility (http://bedtools.readthedocs.org) was used to overlap TopHat2 mappings with mapping quality of at least 20 and defined exons and introns with a minimum overlap fraction of.8.
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