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The SSR markers were assigned to linkage groups (LGs) based on a maximum recombination fraction of 0.34 and a minimum logarithm of odds (LOD) score of 6.0.
First, SDA markers were grouped using a minimum logarithm of odds (LOD) score of 14.
The data was analyzed with JoinMap 4.0 using a minimum logarithm of odds (LOD) score of 4.0 [ 38].
Linkage was assigned based on minimum logarithm of odds (LOD) scores of 3.0 and 4.0 for males and females, respectively.
Markers were grouped using a minimum logarithm of odds score of 7 and a maximum recombination fraction of 0.450.
Linkage groups were formed using a minimum logarithm of the odds (LOD) value of 5. Map distances were calculated in centiMorgans (cM) using the Kosambi mapping function [ 41].
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Loci were separated into linkage groups with JMGRP and JMSPL procedures with minimum and maximum logarithm of the odds (LOD) thresholds of 1.0 and 4.0, respectively and LOD increments of 0.1.
A minimum independence logarithm of odds (LOD) score of 11 and a maximum recombination frequency of 0.4 was used to define linkage groups in all map calculations mentioned here on.
Excessive asymmetry was observed in gene families under the strongest stabilising selection, i.e., lower relKa in the singleton ortholog and has a minimum at logarithm of relKa = 0, i.e. in the pairs of paralogs in which the singleton orthologs evolves close to neutrality.
Grouping of markers was performed using a minimum-independence logarithm of odds (LOD) threshold of 5.0.
Visual quality of life (VQOL) and logarithm of minimum angle of resolution visual acuity (VA) were assessed at baseline and 1 year following cataract surgery.
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