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With a view to comparing the cellular morphology of revertants and parental strains, cells were grown in minimum liquid medium with 2% glucose up to mid-log phase and examined under light microscopy at a magnification of × 1000.
To determine the effects of GCRs on the patterns of gene expression, each pair of mutants and parental strains were grown in minimum liquid medium up to mid-log phase and a comparative transcriptomic analysis based on microarray hybridizations was conducted.
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Extracts with inhibitory diameters greater than or equal to 10 mm, were selected to determine the minimum inhibitory concentrations (MICs) by using a microdilution method in liquid medium [ 16].
For minimum inhibition concentration (MIC) test, the inoculum was prepared by growing each microorganism in a liquid medium until a level of approximately 5 × 107 cfu/mL was reached.
Bright Yellow 2; BY-2) suspension cells were cultivated in liquid medium (3% (w/v) sucrose, 4.4 g/L Murashige and Skoog (MS) salts with minimum organics, 0.4 mg/l thiamine, 0.2 mg/L 2,4-dichlorophenoxyacetic acid, 200 mg/L KH2PO4, pH 5.8) at 26 °C in the dark, shaking at 180 rpm.
The generation time (TG) was determined by the formula: T G = Ln 2 / μ esp Eq (2) The minimum inhibitory concentration (MIC) of DBT was carried out using quantitative method of dilution in liquid medium, considered the reference to determine the sensitivity by S. marcescens against the different concentrations of DBT (0.0, 0.5, 1.0 and 2.0 mM).
After a minimum of three generations, age-synchronized larvae were transferred to 10-cm Petri dishes and cultured in liquid medium plus metabolically labeled N (experimental) or N (external standard) bacteria (6 mg/ml).
"If you put a chicken in a liquid medium, it dilutes the flavor," he said.
b 4 Liquid medium (red).
Generally, liquid manure is an anaerobic liquid medium.
Chinchona ledgeriana cells excreted anthraquinones into liquid medium (Robins and Rhodes 1986).
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