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In an additional experiment the minimum lethal temperature and exposure time in hot water to cause 100%% mortality in American signal crayfish (Pacifastacus leniusculus), was determined to be 5 min at 40 °C.
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Importantly, not all temperatures exist in the geographic domain at a given point in time, only those defined by the realized climate space, which relates to lower lethal temperature through minimum ambient temperature and to upper lethal temperature through maximum ambient temperature.
For example, during the non-breeding season, lower lethal temperature and minimum temperature of the coldest month prevent the Field Sparrow (Spizella pusilla) from moving further polewards in North America (Figure 1A) [9] and limit distribution of the Variable Seedeater (Sporophila americana) in cold environments (Figure 1C).
Frost damage for any specific day of the season was assumed to occur when the minimum temperature on that day was below the specific lethal temperature for the phenological stage predicted at that moment (based on phenological models).
Minimum habitat temperature was unrelated to this measure of a species' upper lethal temperature (p = 0.738).
minimum lethal concentration.
Minimum lethal concentration (MLC) of the compounds was also determined.
LD50 is the minimum lethal dose for which we observe a 50% mortality of larvae.
We exposed family groups to a lethal temperature of 34°C and determined individual time-to-death.
The median lethal temperature of the different tissues ranged from −10 to −15 °C (Nee et al. 1995).
The activity of each antimicrobial agent and its combination was assessed in a murine peritonitis model (n=15), using the minimum lethal dose (MLD) of Pa4 as inoculum.
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