As NAST server limits each submission to 500 pyrotags, an individual library was split into smaller subsets comprising 500 pyrotags, aligned with minimum length set at 200 bp, and with other settings kept at their default values [41].
The 75% confidence interval with minimum length was at [1.00e-7 [1.00e-7], and 90% of the unique transcripts had relative abundances less than 1.66e-5.
As a consequence, mono-, di-, and trinucleotide repeats were required to have a minimum length of at least 13, 14, and 15 bp to achieve the minimum score.
All sequences, that covered a minimum length of at least 55% of any Arabidopsis reference sequence, were added to the EST libraries.
If one meets the minimum length of service at each rank, one needs at least 20 years to reach the position of vice-minister.
All unisequences were subjected to search by BLASTX against the Arabidopsis protein database at The Arabidopsis Information Resource (TAIR) for transcript homologs at an E value of less than 1e-5 [ 14], and the accepted annotations had at least one high scoring pair (HSP) of minimum length 30 covering at least 75% of the query sequence with 80% identity.
He suggested considering that the brand names appear in a minimum font size, and for a minimum length of time, at the beginning or end of a show.
Trees of minimum length were found at least three times.
In particular, all repeats of minimum length 100 with at least 80% sequence identity are shown.
For parameter estimation, the frequency of conservation at position 2-12 was found adequate; this choice was based on simulations (data not shown) for finding the minimum length of nucleus at which specificity of match of any miRNA within its family is maximized.
