Sentence examples for minimum isoform fraction from inspiring English sources

Cuffdiff was used to test for statistically significant differences in transcript expression between 2 comparison pairs using multi-read correction –u and –F (minimum isoform fraction) to default value.

The minimum isoform fraction was set to 0.05, the small anchor fraction of spliced reads was set to 0.01, and the minimum and maximum size of introns were set to 30 and 100,000 bp, respectively.

Transcriptome assembly was performed using the Tuxedo pipeline, where reads were mapped to a partial assembly of the B. calyciflorus genome [ 31] via Tophat [ 32], assembled into transcripts using Cufflinks (assembly for individual samples with 0.1 minimum isoform fraction and 0.1 pre-mRNA fraction) and Cuffmerge (combined assembly from all samples).

Default settings were utilized except for the following modifications: the minimum intron length was set to 50 nucleotides, the maximum intron length was set to 250 000 nucleotides, the minimum isoform fraction was set to 0.10, and only uniquely mapping reads were maintained (max-multihits was set to 1).

Isoform fraction across brain regions.

Filtering based on minor isoform fraction excluded 4560 (15%) junctions.

Isoform fraction is calculated as the expression of a single isoform divided by the sum of expression of isoforms assigned to a gene.

Lists the average isoform fraction across brain regions for genes passing expression level filters.

When limiting the gene annotation set to dup5 and dup0 isoforms, most reads originally assigned to minor isoforms and dup4 were assigned to dup5, changing the isoform fractions marginally.

We used sucrose density gradient ultracentrifugation to test if the short isoform fractionated into the detergent resistant membrane fraction (DRM).

Performing a parametric study and to meet a given minimum solar fraction with the lowest cost.