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SpliceMap was run with the same 6 kbp maximum intron size (the minimum intron size is not configurable).
The A. thaliana dataset was run with default parameters except for a minimum intron size of 5 and a maximum intron size of 6000.
To detect splice junctions in this dataset, HMMSplicer was run with a minimum intron size of 10 bp, a maximum intron size of 1 kbp and an anchor size of 6 bp.
TopHat, run with a minimum intron size of 5 bp and a maximum intron size of 6 kbp, was able to locate only 6,346 junctions, less than half the number found by HMMSplicer, with 91.7% (5,820) of these predictions matching TAIR9 annotations (Figure 4a).
The minimum intron size reported in a previous Ensembl release (version 59) of D. rerio was zero nucleotides, with a further 882 introns less than 5 bp.
For all tests, the minimum intron size, n, was set to 40; the maximum intron size, x, was set to 5000; and the minimum chunk size, c, was set to 6, while the MICS, i, was varied.
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In practice, both for steric requirements of intron bending during splicing and due to the need for other signal sequences, minimum intron sizes are likely to be larger (Schwartz et al. 2008).
The minimum and maximum intron sizes were set to 20 bp and 409 600 bp for all approaches.
The minimum, mode, and maximum intron sizes for each fish are given in table 1. Above the 5,000-bp 5,000-bpn figure 1 a, the number of instancutoff each individual size class is very low, causing a great scatter in values, although the trend does not difigure
Based on rice small intron size [ 102], minimum and maximum intron length of 30 and 50,000, respectively, were set.
Other user-provided parameters that limit the supersplat alignment algorithm are the minimum and maximum allowable intron sizes and the minimum overlap of a read on a flanking exon.
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