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The minimum intensity for each peptide feature was set to 500.
In this study, the efficiency is boosted by reducing the spectral information to be considered for the analysis, adding a f0 candidate selection process, and pruning unlikely combinations by applying some constraints, like a minimum intensity for a pattern.
The normalization process need the maximum and minimum intensity for each pixel which can be calculated as: {I}_{mathrm{min}}left x,yright)=Aleft x,yright -Bleft x,yright -Bleft x}_{mathrm{max}}left(x,yright)=Aleft(x,yright)+Bleft(x,yright) (24).
In each experiment the minimum intensity for a 200 µs stimulus required to produce a reliable R2 blink reflex component was determined (motor threshold).
Both peaks reach their minimum intensity for 120° polarization.
Figure 11 shows the development of process dynamics with rising pulse energy as minimum intensity for the aberrated case (top row) and the corrected case (bottom row).
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The mean intensity of spots containing buffer only was calculated for each row of sub-grids, and was taken as the minimum intensity of probes for that subgrid.
A minimum intensity of 5×10 was selected as the threshold for defining a peak as a component.
0 indicates the minimum intensity of that component, while 255 indicates the maximum intensity.
We measured the minimum intensity required for eliciting an H-reflex of 0.2 mV (H-threshold), the amplitude and latency of the H-reflex at MTh and the amplitude of the H-reflex and M-wave at 1.5 MTh.
The minimum intensity threshold for MS/MS fragmentation in the Orbitrap analyzer was 5000 counts and the dynamic exclusion was set to 60 sec with repeat count as one.
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