Sentence examples for minimum insert size from inspiring English sources

Not least, the challenge of eliminating library contamination, error rates of the enzymes used for DNA amplification as well as minimum insert size, adapter and hybridization artifacts etc has to be taken into account while considering epigenome sequencing.

Maximum and minimum insert size of 500 bp and 0 bp respectively were selected for mapping.

PDD should achieve continuous rRNA depletion for inter-probe distances that are at least 20 nt shorter than the minimum insert size of the library.

A minimum insert size of 30 bp and at least 20 bp with quality of phred > 20 were considered for the analysis.

The T. cruzi genome project took the approach of whole-genome shotgun sequencing of clones from libraries of size-selected fragments from a total cell DNA preparation with a minimum insert size of 5 kb.

For each insert size-range analysed, the relative depletion of inter-probe reads was greater for more closely spaced probes, approaching full efficiency as inter-probe distance dropped below the minimum insert size cutoff minus 20 nt.

A set of custom scripts and samtools (v 0.1.18) were used to filter reads to obtain reads that mapped uniquely, allowing up to three mismatches, minimum mapping quality of 10, minimum and maximum insert size of 150 and 1 000 000, respectively.

Each cDNA library had a minimum average insert size ranging from 570 to 1086 bp; the gene discovery rate ranged from 47% to 60% (Table 1).

1 Minimum mean insert size was calculated as the total number of basepairs (after sequence trimming, vector removal, and discarding of sequences less than 150 bp) divided by the number of clones sequenced.

If the genomic source has a haploid length G, the Bernoulli probabilities of S are (4) Where (5) is the minimum admissible insert size and l ≥ m (Eq. 11).

When starting with intact total RNA samples there is no need for contiguous probe coverage of the target, rather a probe spacing of 20 nt less than the minimum anticipated library insert size, or closer, will suffice to obtain even and continuous depletion.