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For a genotype frequency of 10, 20 or 30% the minimum genotype effect that could be detected with a power of 80% was 55%, 35% or 32% respectively.
Therefore, we performed a posterior power calculation to estimate the minimum genotype effect that could be identified with a power of 80%and95%5% confidence [ 32].
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Post-hoc individual comparisons were carried out to evaluate genotype effect at each decibel.
A significant genotype effect was also observed in vGAT puncta (one-way ANOVA, P = 0.0005).
To get the BLUE of each line, we set the genotype effect as fixed.
However, overall, the genotype effect was not significant.
A strong combined genotype effect on each clinical end point was observed.
The F value for genotype effect was less than 1, because Q G) < 0 for the quadratic form associated with the genotype fixed effect.
Genotypes were called for each individual using minimum genotype quality of 20, and a read depth between 3 and 15.
Variations were filtered for a minimum genotype SNP and INDEL quality (20 and 50 respectively).
Genotype effects were not significant.
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