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The minimum genome length analyzed in this study was 4.7 kb (9.4 kb when analyzing both strands), while analysis of pentanucleotides would have required a minimum genome length of 20 kb.
The minimum genome length analyzed in this study was 1.9 kb (3.8 kb when analyzing both strands), which represents an assumption that 95% of tetranucleotide combinations should occur at least 7.5 times.
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We previously have based our minimum genome sequence length for analysis on the assumption that 95% of tetranucleotide combinations should occur at least 10 times [ 18, 25].
We based our minimum genome sequence length on the assumption that 95% of tetranucleotide combinations should occur at least 10 times [ 31].
Block search mode: reversals (inversions) plus block interchange mode; minimum multi-MUM length: 21 bp (closest integer to log2 [1892 Kbp], where 1892 is the average genome length); minimum LCB length: 63 bp (3 x minimum multi-MUM); chromosome type: linear.
A Phamerator database was created using the 61 large genome Spounavirinae phages with minimum, maximum and mean genome length of 127065 bp (A9), 165238 bp (BigBertha) and 147716 bp, respectively (Additional file 2).
To estimate genome length using framework maps, a minimum LOD score of three was chosen.
A minimum LOD score of three was chosen to estimate genome length using framework maps constructed following the methodology previously described in order to avoid overestimation of genome size because of clustered markers.
Furthermore GAAS was used to generate estimates of the community composition using parameters of 50% identity, 50% read coverage, minimum 10e−6 e-value, weighing all hits and normalizing for genome length.
We predicted Rhizophagus minimum genome sizes.
The initial reads were mapped to the human genome (minimum match length 80%; mismatch allowed 10%) using HIVE hexagon [ 55] and the unaligned reads were downloaded (Additional file 1: Figures S1 and S2) and CensuScope was run to obtain the taxonomic composition of the non-human reads.
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