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Resolution of individual products within the multiplex required a minimum fluorescence signal of not less than 1000 units per PCR product.
The data were normalized based on the maximum fluorescence signal values for dimethyl sulfoxide (DMSO -treated wells (no inhibition by compounDMSO -treatednimum fluorescence signal values for wells conoainhibitionhighest concentration of inhibytor compoundcompounds.
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For minimum fluorescence ratio signal, the solution was nominally Ca2+-free and contained 10 mM EGTA.
The combination of vector/temperature giving the minimum detectable fluorescence signal is chosen for the first round of evolution (Fig. 6A, 2.3).
But that is why we performed two different measurements, including one based on tumor area which is defined by the pixels reaching a minimum threshold fluorescence signal from the glowing tumor cells and thus will be much less sensitive to such attenuation.
The fluorescence signal was measured as the difference of the maximum and minimum intensity within a defined region of interest (ROI) around each cell.
the minimum fluorescence in the dark-adapted state.
This property facilitates easy calibration of the fluorescence signal.
We also examined fluorescence signal of MSCs by a fluorescence microscope.
The fluorescence signal was observed using confocal microscopy.
(c) The fluorescence signal of in vivo gastric cancer (pseudocolor).
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