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An intensity threshold to image residual NIR fluorescence was set based on 80% of the minimum fluorescence emission rate from the tumor image for each mouse.
By using these data, a threshold of 80% of the minimum fluorescence emission rate of the primary tumor was established as capable of identifying mice with biopsy-proven microscopic residual disease in the tumor bed (Fig. 3a).
The tumor beds were imaged and classified as positive or negative for residual NIR fluorescence based on 80% of the minimum fluorescence emission rate of the primary sarcoma, as determined previously.
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We propose two ICT minima states out of six conceivable twisted emitting S1 minima as the most likely emitting species for the fluorescence emission.
It follows that aberrations that cause a non-zero value of this minimum intensity are the most detrimental, as they inhibit fluorescence emission even at the centre of the depletion focus.
Fluorescence emission of the hybrid silicon-conjugated polymer nanocomposites is steeply quenched by cytochrome c, and the minimum detection concentration for the redox-active protein is found to be 50 nM.
Therefore, we carried out fluorescence emission and excitation scans to determine the most ideal excitation and emission spectra for each drug with minimum or no overlap of emission in the two drugs' spectra upon detection.
two-photon fluorescence emission.
Figure 4 Fluorescence emission spectra.
Figure 6 Fluorescence emission spectra of samples.
Figure 6 Fluorescence emission spectra of PHPP.
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