Sentence examples for minimum flanking sequence from inspiring English sources
Therefore in this mode, the minimum flanking sequence was 30 bp, the minimum to identify a fusion.
A minimum of 50 bp flanking sequence upstream and downstream of the SNP site is required for submission [ 28].
Of all SNPs reported, 12,339 had sufficient flanking sequence (minimum 30 nt on either side of variable site) for primer design and scoring.
Hits of sufficient length, typically at least 100 bp, were extracted from the genome along with a minimum of 500 bp of flanking sequence using custom PERL scripts.
Illumina recommends that SNPs to be targeted in the Goldengate assay should have a minimum of 50 bases of flanking sequence free of polymorphism on each side: the high and clustered nature of polymorphisms meant that only 5% of our SNPs fulfilled this criterion.
This procedure resulted in 704,687 SNPs, among them, 702,590 had a minimum of 100 nucleotides in each flanking sequence of SNPs.
The filtering criteria were implemented in PERL and were: a) 50 bp of uninterrupted flanking sequence, b) a minimum of 8 reads mapped to the contig containing the SNP and c) at least 15% minimum frequency for the minor allele.
Only microsatellite sequences that were polymorphic in the source species and had sufficient flanking sequence for primer design were considered (i.e. a minimum of 30 bp of flanking sequence on either side of the repeat motif).
The position of the great tit SNPs on the zebra finch, chicken and green anole lizard genomes was predicted based on the alignment of 23 to 150 bases of flanking sequence to the respective genome sequence using the default parameters in blastn (with setting a minimum expectation value (e-value) of 10−5).
The inserted loci for OsOPT3 (OsGT1) and OsOPT9 were confirmed by flanking sequence method.
