Sentence examples for minimum expression threshold from inspiring English sources
To select a useful minimum expression threshold for validation of this method and confidant correction of gene models, we evaluated the number of FDM sequences for all gene models expressed in our data set at a range of expression levels (Figure 2).
We also set a minimum expression threshold of two reads per exon.
In total, 26,973 genes with the minimum expression threshold of FPKM > 3 were identified in at least one RNA-Seq library.
Given expression data and a minimum expression threshold, GIMME calculates a normalized gene "score" for each reaction by averaging the expression values of all genes that appear in the GPR for the reaction.
Additional file 4: Contains a worksheet listing the 8,040 genes that met the minimum expression threshold in both filter and suspension experiments, and were thus included in PCA and MDS analyses.
Using UniHI, users can filter interacting proteins by requiring a minimum expression threshold, and the PPI network retrieved from UniHI can be reduced to include only highly expressed proteins or extended to include lowly expressed proteins.
Unregulated (control) transcripts were assigned as those passing minimum expression thresholds and having expression changes of less than 0.3 Log2 units (1.23-fold) in both starvation- and DCE- synchronized cells.
With the minimum expression level threshold of two reads, transcripts were sorted in ascending order and arranged in bins of size 300.
With this caveat in mind, we sought to minimize the effect of binding bias on our analysis by: (a) employing a minimum 1.5-fold change, expression threshold for expression differences to be considered significant; and (b) analysing only genes with expression information on all available replicate spots.
In order to use only transcripts expressed above the noise level in the analysis, a threshold of minimum expression was fixed as the mean plus two times the standard deviation of pseudo-spots on the array that contained no cDNA sequences.
There are two separate questions that might be addressed by a read count threshold: a minimum expression level for biologically significant effects and a minimum count for statistically reliable analysis.
