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In addition to the organ marker genes, we identified a multitude of genes (6075 IDs) that were expressed at comparable levels (with a minimum expression of 6, and a range<1) in all five organs (Table S1).
Since 100 RPKE was determined as the minimum expression for gene detection, Tiling Assembly identified 20,230 expressed annotated genes in rice aleurone.
Filtering by a minimum expression level obscures the influence of extreme variability of low expressed genes.
A t-test using the Benjamini-Hochberg multiple hypothesis correction (p < 0.01) and a 2-fold minimum expression change was used to identify 140 genes that were differentially expressed between cervical cancer and normal cervix.
A 2.0× fold minimum expression difference was used in previous studies (Song et al. 2013; Paschold et al. 2012).
Plan Ceibal is reduced to its minimum expression, and a new divide in access and use of educational technologies is increasing.
Fig. 1 Genome-wide analysis of ASE in F1 at (left) non- and (right) water-stress conditions (for 2× fold minimum expression).
Log-2 expression ratios were filtered to find genes with a 3-fold minimum expression ratio.
Gene probes were filtered according to the following criteria: maximum expression value/minimum expression value ≥1.7, minimum expression variation (maximum value−minimum value) ≥9, expression value greater than 20 or less than 20,000.
For samples that showed no expression of the test gene, the value of minimum expression was used for statistical analysis.
RPL34 expression peaked at 4 DPI in resistant plants while susceptible plants showed minimum expression at 4 DPI.
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