Sentence examples for minimum essential media from inspiring English sources
Monkey brain and liver and tick pools were sonicated in 600 μL of Minimum Essential Media (GIBCO/BRL, Life Technologies, Grand Island, NY, USA), and 400 μL of media was added to the homogenate.
Eagle's minimum essential media.
Fibrinogen stock solutions (110 mg/mL) were made by dissolving Fb in one-part 10× Eagle's minimum essential media (EMEM) and nine parts HFIP.
After 24 h, the growth medium was replaced with 1.0 mL of minimum essential media (MEM), and 100 µL of MTT (5 mg/mL in PBS).
Human epithelial colorectal adenocarcinoma cells (Caco-2) were grown in minimum essential media (MEM; Thermo Fisher Scientific) supplemented with 20% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin streptomycin (Thermo Fisher Scientific).
Cells were routinely grown in minimum essential media (MEM + GlutaMax, Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Gibco) and 1% penicillin-streptomycin (Gibco) in 75-cm2 flasks and maintained in a 37°C humidified incubator containing 5% CO2.
Cells were maintained in growth media consisting of Minimum Essential Media-Alpha (MEM-α), 10% fetal bovine serum (FBS), and 1% penicillin streptomycin. Stable both firefly and renilla luciferase-overexpressing SKOV3 cells were prepared using lentivirus vectors according to the manufacturer's protocols (Cell Biolabs, San Diego, CA).
L6-GLUT4myc rat myoblasts (kindly provided by Amira Klip, Toronto, Canada) were maintained in α-minimum essential media (MEM) with 10% FBS.
The isolated cells were suspended in alpha-minimum essential media (α-MEM; Invitrogen, USA), supplemented with 10% fetal bovine serum (Invitrogen, USA), and plated onto a 9.6 cm culture plate.
P19 embryonal carcinoma cells (American Type Culture Collection, VA, USA) were cultured as previously described [ 93] in α-minimum essential media supplemented with 5% cosmic calf serum (Hyclone, UT, USA) and 5% fetal bovine serum (Cansera, Rexdale, ON, Canada).
U87 cells were cultured in α-Minimum Essential Media (α-MEM) supplemented with 10% heat-inactivated fetal bovine serum at 37°C in an incubator comprising 5% CO2 and 95% air.
