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The ELISA minimum detectable doses were 0.03 μg/ml, 10 ng/ml, 20 ng/ml and 50 ng/ml.
The minimum detectable doses were experimentally determined as 1.0 pg/mL, 1.6 pg/mL, and 2.0 pg/mL for IL-6, TNF-α, and IFN-γ, respectively.
The minimum detectable doses of TIMP-1, TIMP-2 and TIMP-4 were 0.08 ng/mL, 0.011 ng/mL and 4.91 pg/mL respectively.
The minimum detectable doses of IL-17A and IL-17 F were 0.23 pg/ml and 31.3 pg/ml, respectively, and the intra-assay coefficient of variation was <10%.
The minimum detectable doses of IL-12p70, IL-4, IL-6, IL-10 and IFNγ with these assay kits were 15, 4, 1, 4 and 4 pg/ml, respectively.
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The Monte Carlo simulation and minimum detectable dose are also discussed and demonstrated the reliability of using the TLD-600 approach.
The minimum detectable dose of VEGF is <5 pg/ml, and 100% cross-reactivity with human VEGF-121 and complete parallelism with human VEGF-165.
using a Parameter cAMP kit (R&D Systems; Minneapolis, MN) with a mean minimum detectable dose of 1.50 pmol/mL (manufacturer protocol).
Per manufacturer's instructions, the minimum detectable dose was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
The minimum detectable dose of sRAGE was 4 pg/ml.
The minimum detectable dose of IGF-I was 1.3 ng/mL.
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