The programs were run using default parameters except for Cufflinks option "-j" that was set to 0.3 in order to increase the minimum depth of coverage in the intronic region and IsoLasso option "-c" that was increased to 5 to adjust the minimum number of clustered reads.
Validation was performed for all CpG sites in ESC and NPC that were covered (minimum depth of 10) in both the RRBS and the BS-seq datasets.
In order to check the applicability of the proposed equations for minimum depth of cover in practical problems, a series of 3D finite element analyses with more realistic idealization of the railway superstructure components and the lateral slope of bridge embankment were carried out to control the out-of-plane buckling in the steel plates.
The minimum depth of cover in which all of the criteria were simultaneously fulfilled was chosen as the minimum depth of soil cover for a specific bridge structure.
A 0-1 ILP formulation for optimally solving the special case of minimum depth adder graphs in a graph-based fashion was recently proposed [26].
They found that the most useful factors for determining if a particular species had an elevated risk of extinction was its maximum body size, the minimum depth of water in which it lived, and the range of depth with larger species and those that swim in shallower waters having the largest risk.
For example the area of the Gorringe Bank (minimum depth less than 50 m) in front to the Strait of Gibraltar is characterised by a dense assemblage of Paramuricea clavata and, on its upper portion, by a dense meadow of Laminaria ochroleuca De La Pylaie, 1824 [44].
The probability that a species is threatened increases by 1.2% for each 10 cm increase in maximum body length, and decreases by 10.3% for each 50 m deepening in the minimum depth limit of species.
However, due to the dimensions of the formulas for calculating the minimum depth of cover that must be in meters, an exponential function (D h ) was chosen to describe the change in the minimum depth of cover versus the effective span.
For a soil-steel railway bridge, the minimum depth of cover must be defined in such a way to prevent the uneven settlements.
